肺炎双球菌野生型和氨基蝶呤抗性突变体的二氢叶酸还原酶
Dihydrofolate reductases from the wild type and aminopterin-resistant mutants of Diplococcus pneumoniae.
作者信息
Trombe M C, Sicard A M
出版信息
J Bacteriol. 1975 Mar;121(3):766-70. doi: 10.1128/jb.121.3.766-770.1975.
Abstract
Dihydrofolate reductase from the wild type and aminopterin-resistant mutants of Diplococcus pneumoniae has been compared. Specific activity, optimum pH, Km, thermal stability, and inhibition by aminopterin are identical for both strains. Aminopterin resistance for such mutants is, therefore, not due to an alteration of the dihydrofolate reductase.
摘要
已对肺炎双球菌野生型和氨基蝶呤抗性突变体的二氢叶酸还原酶进行了比较。两种菌株的比活性、最适pH、米氏常数、热稳定性以及对氨基蝶呤的抑制作用均相同。因此，此类突变体的氨基蝶呤抗性并非由于二氢叶酸还原酶的改变所致。
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