Department of Biology, Faculty of Arts and Science, Bülent Ecevit University, Zonguldak, Turkey.
Arch Insect Biochem Physiol. 2013 May;83(1):15-24. doi: 10.1002/arch.21089. Epub 2013 Mar 11.
Plant allelochemicals are nonnutritional chemicals that interfere with the biology of herbivores. We posed the hypothesis that ingestion of a glycoalkaloid allelochemical, α-solanine, impairs biological parameters of greater wax moths Galleria mellonella. To test this idea, we reared wax moths on artificial diets with 0.015, 0.15, or 1.5 mg/100 g diet of α-solanine. Addition of α-solanine to the diet affected survival of seventh-instar larvae, pupae, and adults; and female fecundity and fertility. The diet containing the highest α-solanine concentration led to decreased survivorship, fecundity, and fertility. The diets supplemented with α-solanine led to increased malondialdehyde and protein carbonyl contents in midgut and fat body and the effect was dose-dependent. Dietary α-solanine led to increased midgut glutathione S-transferase activity and to decreased fat body glutathione S-transferase activitiy. We infer from these findings that α-solanine influences life history parameters and antioxidative enzyme activities in the midgut and fat body of G. mellonella.
植物化感物质是非营养性化学物质，会干扰草食动物的生物学特性。我们提出了一个假设，即摄入糖苷生物碱化感物质α-茄碱会损害大蜡螟 Galleria mellonella 的生物学参数。为了验证这个想法，我们在人工饲料中饲养蜡螟，饲料中含有 0.015、0.15 或 1.5 mg/100 g 的 α-茄碱。α-茄碱添加到饮食中会影响 7 龄幼虫、蛹和成虫的存活率；以及雌虫的繁殖力和生育率。含有最高浓度 α-茄碱的饮食导致存活率、繁殖力和生育率下降。添加 α-茄碱的饮食会导致中肠和脂肪体中的丙二醛和蛋白质羰基含量增加，且呈剂量依赖性。饮食中的 α-茄碱会增加中肠谷胱甘肽 S-转移酶的活性，同时降低脂肪体谷胱甘肽 S-转移酶的活性。我们从这些发现推断，α-茄碱会影响 G. mellonella 中肠和脂肪体的生活史参数和抗氧化酶活性。
