State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Guangzhou, China.
J Transl Med. 2013 Mar 8;11:60. doi: 10.1186/1479-5876-11-60.
Tumor-derived cytokines and their receptors usually take important roles in the disease progression and prognosis of cancer patients. In this survey, we aimed to detect the expression levels of MIF and CXCR4 in different cell populations of tumor microenvironments and their association with survivals of patients with esophageal squamous cell carcinoma (ESCC).
MIF and CXCR4 levels were measured by immunochemistry in tumor specimens from 136 resected ESCC. Correlation analyses and independent prognostic outcomes were determined using Pearson's chi-square test and Cox regression analysis.
The expression of CXCR4 in tumor cells was positively associated with tumor status (P = 0.045) and clinical stage (P = 0.044); whereas the expression of CXCR4 in tumor-infiltrating lymphocytes (TILs) and the expression of MIF in tumor cells and in TILs were not associated with clinical parameters of ESCC patients. High MIF expression in tumor cells or in TILs or high CXCR4 expression in tumor cells was significantly related to poor survival of ESCC patients (P < 0.05). Multivariate analysis showed that the expression of MIF or CXCR4 in tumor cells and the expression of MIF in TILs were adverse independent factors for disease-free survival (DFS) and overall survival (OS) in the whole cohort of patients (P < 0.05). Furthermore, the expression of MIF and CXCR4 in tumor cells were independent factors for reduced DFS and OS in metastatic/recurrent ESCC patients (P < 0.05). Interestingly, the expressions of MIF and CXCR4 in tumor cells and in TILs were significantly positively correlated (P < 0.05), and the combined MIF and CXCR4 expression in tumor cells was an independent adverse predictive factor for DFS and OS (P < 0.05).
The expressions of MIF and CXCR4 proteins in tumor cells and TILs have different clinically predictive values in ESCC.
肿瘤来源的细胞因子及其受体通常在癌症患者的疾病进展和预后中起重要作用。在本研究中，我们旨在检测肿瘤微环境中不同细胞群中 MIF 和 CXCR4 的表达水平及其与食管鳞状细胞癌（ESCC）患者生存的关系。
采用免疫组织化学法检测 136 例 ESCC 切除标本中 MIF 和 CXCR4 的表达。采用 Pearson 卡方检验和 Cox 回归分析进行相关性分析和独立预后结果。
肿瘤细胞中 CXCR4 的表达与肿瘤状态（P = 0.045）和临床分期（P = 0.044）呈正相关；而肿瘤浸润淋巴细胞（TILs）中 CXCR4 的表达以及肿瘤细胞和 TILs 中 MIF 的表达与 ESCC 患者的临床参数无关。肿瘤细胞中 MIF 表达高或 TILs 中 MIF 表达高或肿瘤细胞中 CXCR4 表达高与 ESCC 患者的生存不良显著相关（P < 0.05）。多因素分析显示，肿瘤细胞中 MIF 或 CXCR4 的表达以及 TILs 中 MIF 的表达是全队列患者无病生存（DFS）和总生存（OS）的不良独立因素（P < 0.05）。此外，肿瘤细胞中 MIF 和 CXCR4 的表达是转移性/复发性 ESCC 患者 DFS 和 OS 降低的独立因素（P < 0.05）。有趣的是，肿瘤细胞和 TILs 中 MIF 和 CXCR4 的表达呈显著正相关（P < 0.05），肿瘤细胞中 MIF 和 CXCR4 的联合表达是 DFS 和 OS 的独立不良预测因素（P < 0.05）。
肿瘤细胞和 TILs 中 MIF 和 CXCR4 蛋白的表达在 ESCC 中有不同的临床预测价值。
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