Department of Pathology, Faculty of Veterinary Medicine, Univeristy of Belgrade, Bulevar oslobodjenja 18, Belgrade, Serbia.
BMC Vet Res. 2013 Mar 6;9:45. doi: 10.1186/1746-6148-9-45.
This paper describes liver cirrhosis in 35 fallow deer infected with the giant liver fluke, as well as the distribution, origin, and role of myofibroblasts in its development.
In liver of infected deer, stripes of connective tissue are wound around groups of degenerated and regenerated liver lobuli. In the connective tissue, lymphocytes and macrophages which often contain parasite hematin are also present. The walls of the bile ducts are thickened, the epithelium multiplied with mucous metaplasia, and desquamated cells, parasite eggs and brown pigment are present in their lumen.In the livers with cirrhosis, immunopositivity to α-SMA and desmin was observed in cells in portal and septal spaces, at the edge between fibrotic septa and the surrounding parenchyma and in perisinusoidal spaces. These cells vary in size, they are round, oval, spindle-shaped or irregular in shape, similar to vascular smooth muscle cells. The derangement of epithelial-mesenchymal interactions detected in chronic cholangiopathies is most probably the pro-fibrogenic mechanism in liver cirrhosis of fallow deer (Dama dama) infected with the giant liver fluke (Fascioloides magna).
Myofibroblasts, especially hepatic stellate cells (HSCs), play an important role in the synthesis of extracellular matrix components in the development of parasitic fibrosis and cirrhosis in the liver of fallow deer.
本文描述了感染巨型片形吸虫的 35 只赤鹿的肝硬化，并介绍了肌成纤维细胞在其发生发展中的分布、起源和作用。
在感染鹿的肝脏中，结缔组织条纹缠绕在变性和再生的肝小叶群周围。在结缔组织中，也存在淋巴细胞和巨噬细胞，它们通常含有寄生虫血素。胆管壁增厚，上皮细胞增生伴有粘液化生，管腔中存在脱落细胞、寄生虫卵和棕色色素。在肝硬化的肝脏中，α-SMA 和结蛋白在门脉和间隔空间、纤维隔边缘与周围实质之间以及窦周空间的细胞中呈免疫阳性。这些细胞大小不一，呈圆形、椭圆形、梭形或不规则形，类似于血管平滑肌细胞。在慢性胆管病变中检测到的上皮间充质相互作用的紊乱，很可能是感染巨型片形吸虫的赤鹿（Dama dama）肝硬化的促纤维化机制。
肌成纤维细胞，特别是肝星状细胞（HSCs），在寄生虫性纤维化和赤鹿肝脏肝硬化中细胞外基质成分的合成中发挥重要作用。
