Division of Food Chemistry, Osaka Prefectural Institute of Public Health, 1-3-69 Nakamichi, Higashinari-ku, Osaka 537-0025, Japan.
Food Microbiol. 2013 May;34(1):29-37. doi: 10.1016/j.fm.2012.11.010. Epub 2012 Nov 28.
The Bacillus cereus emetic toxin cereulide causes foodborne intoxication, which may occasionally result in severe disease, and even death. To differentially diagnose the emetic-type of foodborne disease caused by B. cereus and assess the safety of commercial food, we developed a rapid method to quantitate cereulide. This method was combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis for the extraction of cereulide from food using a normal-phase silica gel cartridge. The limits of detection and quantification were 0.1 and 0.5 ng of cereulide ml(-1), respectively. Spiked cereulide was reproducibly recovered with over 67% efficiency from nine diverse foods implicated in cereulide food poisoning. The recovery rate, reproducibility, and intermediate precision for this single laboratory validation using boiled rice were 87.1%, 4.4%, and 7.0%, respectively. Further, we detected a wide range of cereulide concentrations in leftover food and vomitus samples from two emetic foodborne outbreaks. LC-MS/MS analysis correlated closely with those acquired using the HEp-2 cell assay, and quantitated cereulide from 10 food samples at least five times faster than the bioassay. This new method will provide clinicians with an improved tool for more rapidly and quantitatively determining the presence of cereulide in food and diagnosing food poisoning caused by cereulide.
蜡样芽胞杆菌呕吐毒素cereulide 可引起食源性中毒，偶尔可能导致严重疾病，甚至死亡。为了对蜡样芽胞杆菌引起的呕吐型食源性疾病进行鉴别诊断，并评估商业食品的安全性，我们开发了一种定量检测 cereulide 的快速方法。该方法结合液相色谱-串联质谱（LC-MS/MS）分析，采用正相硅胶小柱从食品中提取 cereulide。检测限和定量限分别为 0.1 和 0.5 ng cereulide ml(-1)。在涉及 cereulide 食物中毒的 9 种不同食品中，添加的 cereulide 可重复性地以超过 67%的效率回收。在单一实验室对米饭进行验证时，回收率、重现性和中间精密度分别为 87.1%、4.4%和 7.0%。此外，我们在两起呕吐型食源性暴发的剩余食物和呕吐物样本中检测到了广泛的 cereulide 浓度。LC-MS/MS 分析与 HEp-2 细胞测定法密切相关，并且比生物测定法至少快 5 倍定量检测了 10 个食品样品中的 cereulide。这种新方法将为临床医生提供一种更快速、更定量地确定食品中 cereulide 存在和诊断 cereulide 引起的食物中毒的改进工具。
