School of Stomatology, China Medical University, 117 Nanjing North Sreet, Shenyang, 110002, China.
Mater Sci Eng C Mater Biol Appl. 2013 May 1;33(4):2113-21. doi: 10.1016/j.msec.2013.01.033. Epub 2013 Jan 20.
In comparison with titanium and its alloys, Zr61Ti2Cu25Al12 (ZT1) bulk metallic glass (BMG) manifests a good combination of high strength, high fracture toughness and lower Young's modulus. To examine its biocompatibility required for potential use in dental implants, this BMG was used as a cell growth subtract for three types of cell lines, L929 fibroblasts, human umbilical vein endothelial cells (HUVEC), and osteoblast-like MG63 cells. For a comparison, these cell lines were in parallel cultured and grown also on commercially pure titanium (CP-Ti) and Ti6-Al4-V alloy (Ti64). Cellular responses on the three metals, including adhesion, morphology and viability, were characterized using the SEM visualization and CCK-8 assay. Furthermore, real-time RT-PCR was used to measure the activity of integrin β, alkaline phosphatase (ALP) and type I collagen (COL I) in adherent MG63 cells. As indicated, in all cases of three cell lines, no significant differences in the initial attachment and viability/proliferation were found between ZT1, CP-Ti, and Ti64 until 5d of incubation period. It means that the biocompatibility in cellular response for ZT1 BMG is comparable to Ti and its alloys. For gene expression of integrin β, ALP and COL I, mRNA level from osteoblast cells grown on ZT1 substrates is significantly higher than that on the CP-Ti and Ti64. It suggests that the adhesion and differentiation of osteoblasts grown on ZT1 are even superior to those on the CP-Ti and Ti64 alloy, then promoting bone formation. The good biocompatibility of ZT1 BMG is associated with the formation of zirconium oxide layer on the surface and good corrosion-resistance in physiological environment.
与钛及其合金相比，Zr61Ti2Cu25Al12（ZT1）块体金属玻璃（BMG）表现出高强度、高断裂韧性和较低杨氏模量的良好结合。为了研究其作为牙科植入物潜在应用的生物相容性，将该 BMG 用作三种细胞系（L929 成纤维细胞、人脐静脉内皮细胞（HUVEC）和成骨样 MG63 细胞）的细胞生长底物。为了进行比较，这些细胞系也在商业纯钛（CP-Ti）和 Ti6-Al4-V 合金（Ti64）上进行平行培养和生长。使用 SEM 可视化和 CCK-8 测定法对三种金属上的细胞反应，包括粘附、形态和活力进行了表征。此外，使用实时 RT-PCR 测量了粘附的 MG63 细胞中整合素 β、碱性磷酸酶（ALP）和 I 型胶原（COL I）的活性。结果表明，在所有三种细胞系的情况下，在孵育 5d 期间，ZT1、CP-Ti 和 Ti64 之间在初始附着和活力/增殖方面没有发现明显差异。这意味着 ZT1 BMG 在细胞反应中的生物相容性可与 Ti 及其合金相媲美。对于整合素 β、ALP 和 COL I 的基因表达，在 ZT1 基质上生长的成骨细胞的 mRNA 水平明显高于 CP-Ti 和 Ti64。这表明，在 ZT1 上生长的成骨细胞的粘附和分化甚至优于 CP-Ti 和 Ti64 合金，从而促进骨形成。ZT1 BMG 的良好生物相容性与表面形成氧化锆层和在生理环境中的良好耐腐蚀性有关。
