Gene. 2013 Aug 10;525(2):151-4. doi: 10.1016/j.gene.2013.03.006. Epub 2013 Mar 14.
Applying my experience in microbial genetics, especially in the genetic transformation/transduction of Bacilus subtilis bacteria, I decided around 1956 to develop a similar system for eukaryotic, especially human cell cultures. I believed it would permit the development of clinical applications for replacing defective genes to treat or cure some of the genetic diseases.
应用我在微生物遗传学方面的经验,特别是在枯草芽孢杆菌的遗传转化/转导方面的经验,我在 1956 年左右决定开发一个类似的系统用于真核生物,特别是人类细胞培养。我相信这将允许开发临床应用来替代有缺陷的基因,以治疗或治愈一些遗传疾病。