Gunin A G, Gordon D S
Arkh Anat Gistol Embriol. 1990 Jan;98(1):68-70.
To 10 non-inbred white mature rats 24 h before sacrifice 7.0 ml of Indian ink colloid solution has been injected intraperitoneally. Histochemical reactions of Falk-Hillarp (catecholamines, serotonin) have been performed on nonfixed cryostat slices of the uterus, those of Cross, Even, Rost (histamine) against non-specific esterase and acid phosphatase. Before carrying out these reactions, the slices have been examined under luminescent microscope LUMAM-I3 by means of luminescent and usual illumination in order to reveal cells, possessing autoluminiscence and containing phagocyted Indian ink particles. Presence of autoluminescence, phagocyted material, catecholamines and serotonin, histamine, nonspecific esterase, acid phosphotase, prostaglandin E2 in the same granular cells of the rat endometrium have been stated. All cellular properties revealed are specific for macrophages. A conclusion is made that granular biogenic amine-containing cells of endometrium can be considered as belonging to the system of mononuclear phagocytes.
在处死前24小时，给10只非近交系白色成年大鼠腹腔注射7.0毫升印度墨水胶体溶液。对子宫的未固定低温切片进行了Falk-Hillarp（儿茶酚胺、5-羟色胺）组织化学反应，对Cross、Even、Rost（组胺）进行了针对非特异性酯酶和酸性磷酸酶的组织化学反应。在进行这些反应之前，通过荧光和普通照明在LUMAM-I3荧光显微镜下检查切片，以发现具有自发荧光并含有吞噬的印度墨水颗粒的细胞。已证实大鼠子宫内膜的相同颗粒细胞中存在自发荧光、吞噬物质、儿茶酚胺、5-羟色胺、组胺、非特异性酯酶、酸性磷酸酶、前列腺素E2。所揭示的所有细胞特性都是巨噬细胞特有的。得出的结论是，子宫内膜中含颗粒生物胺的细胞可被认为属于单核吞噬细胞系统。
