Dipartimento di Chimica "Ciamician", Università di Bologna, Via Selmi 2, I-40126 Bologna, Italy.
Beilstein J Org Chem. 2013;9:417-24. doi: 10.3762/bjoc.9.44. Epub 2013 Feb 22.
A physical hydrogel prepared with the low-molecular-weight hydrogelator (LMWHG) CH2(C3H6CO-L-Phe-D-Oxd-OH)2 and water/ethanol mixture was applied as a potential "Trojan Horse" carrier into cells. By SEM and XRD analysis we could demonstrate that a fibrous structure is present in the xerogel, making a complex network. The gelator is derived from α-amino acids (Thr, Phe) and a fatty acid (azelaic acid) and is biocompatible: it was dosed to IGROV-1 cells, which internalized it, without significantly affecting the cell proliferation. To check the internalization process by confocal microscopy, fluorescent hydrogels were prepared, introducing the fluorescent dansyl moiety into the mixture.
一种由低分子量水凝胶剂 (LMWHG) CH2(C3H6CO-L-Phe-D-Oxd-OH)2 和水/乙醇混合物制备的物理水凝胶被用作潜在的“特洛伊木马”载体进入细胞。通过 SEM 和 XRD 分析，我们可以证明在干凝胶中存在纤维结构，形成复杂的网络。凝胶剂来源于α-氨基酸（苏氨酸、苯丙氨酸）和脂肪酸（壬二酸），具有生物相容性：它被给予 IGROV-1 细胞，细胞将其内化，而不会显著影响细胞增殖。为了通过共聚焦显微镜检查内化过程，制备了荧光水凝胶，将荧光丹磺酰部分引入混合物中。
