Ohno Y, Okazaki M, Hara I
Department of General Education, Tokyo Medical and Dental University, Chiba Prefecture, Japan.
Biochem Cell Biol. 1990 Jan;68(1):376-9. doi: 10.1139/o90-052.
The activity of phospholipase D from the Savoy cabbage on alkyl phosphorylcholines containing alkyl groups of C14 to C22 was examined. Apparent Km and maximal velocity values in the presence of diethyl ester were 3.6-8.3 mM and 0.14-0.29 mumol/(min.mg protein), respectively. The maximal velocities for alkyl phosphorylcholine homologues were lower than the value for pig phosphatidylcholines, but higher than those for lysophosphatidylcholines and synthetic phosphatidylcholine with saturated acyl chains. Results suggest that solubilities of the substrates in the organic solvent play an important role in activity observed with phospholipase D.
研究了皱叶甘蓝中磷脂酶D对含有C14至C22烷基的烷基磷酰胆碱的活性。在二乙酯存在下的表观Km值和最大速度值分别为3.6 - 8.3 mM和0.14 - 0.29 μmol/(min·mg蛋白质)。烷基磷酰胆碱同系物的最大速度低于猪磷脂酰胆碱的值，但高于溶血磷脂酰胆碱和具有饱和酰基链的合成磷脂酰胆碱的值。结果表明，底物在有机溶剂中的溶解度对磷脂酶D所观察到的活性起着重要作用。
