沙丁胺醇多克隆抗体的制备及间接竞争酶联免疫分析法的建立
[Preparation of salbutamol polyclonal antibodies and development of indirect competitive enzyme-linked immunoassay].
作者信息
Zhang Shujie, Lei Yajing, Xu Xiaoqian, Shi Weixing, Chen Shuqing
机构信息
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
出版信息
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2013 Jan;42(1):19-24.
OBJECTIVE
To prepare the antibodies against salbutamol (SAL) with high sensitivity and to develop an indirect competitive enzyme-linked immunoassay (ic-ELISA) for fast detection of SAL.
METHODS
The New Zealand white rabbits were immunized with SAL in a small dose and long period mode. The method of ic-ELISA was optimized and adopted for the detection of a series of SAL samples, then the standard curve of SAL was established. The precision and the recoveries of the method were determined.
RESULTS
The antibodies with high sensitivity towards SAL were prepared with a IC50 of 12.21 ng/ml. The ic-ELISA method for SAL measurement was established, the recoveries of measurement was between 95%-105% and the CV was <3%.
CONCLUSION
The antibodies against salbutamol have been prepared and an indirect competitive enzyme-linked immunoassay for fast and specific detection of SAL has been developed.
目的
制备高灵敏度的沙丁胺醇（SAL）抗体，并建立一种间接竞争酶联免疫吸附测定法（ic-ELISA）用于快速检测SAL。
方法
采用小剂量、长时间的方式用SAL免疫新西兰白兔。对ic-ELISA方法进行优化，并用于检测一系列SAL样品，然后建立SAL的标准曲线。测定该方法的精密度和回收率。
结果
制备了对SAL具有高灵敏度的抗体，其IC50为12.21 ng/ml。建立了用于测定SAL的ic-ELISA方法，测定回收率在95%-105%之间，变异系数<3%。
结论
已制备出抗沙丁胺醇抗体，并建立了一种用于快速、特异性检测SAL的间接竞争酶联免疫测定法。
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