Liu Wei, Zhao Chengbiao, Zhang Yulan, Lu Shengxin, Liu Jinting, Xi Rimo
The School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong 250100, People's Republic of China.
J Agric Food Chem. 2007 Aug 22;55(17):6829-34. doi: 10.1021/jf070620k. Epub 2007 Jul 24.
Nitrofurans are used widely to treat animal diseases and were identified as the major compounds in many worldwide drug residue violations. To develop a rapid and convenient detection method to measure the residue of nitrofurantoin, we designed an immunogen and prepared a polyclonal antibody to develop an immunoassay in this study. The antibodies obtained were characterized by an indirect cELISA method and showed excellent specificity and sensitivity with IC50 of 3.2 ppb and no cross-reaction with most related species and compounds. Considering that nitrofurans often are used illegally to feed animals through drinking water, we measured the residue of nitrofurantoin in water spiked by the drug. The recovery rates are in the ranges of 88-103% for interassay and 90-103% for intra-assay. The CVs are in the ranges of 3.1-11.4% for interassay and 2.7-6.2% for intra-assay. The detection limit was determined to be 0.2 ppb. The immunoassay developed in this study is suitable to be used as a screening method to detect residues of nitrofurantoin in drinking water for animals.
硝基呋喃被广泛用于治疗动物疾病，并且在许多全球范围内的药物残留违规事件中被确定为主要化合物。为了开发一种快速便捷的检测方法来测定呋喃妥因残留量，我们在本研究中设计了一种免疫原并制备了多克隆抗体以开发一种免疫测定法。通过间接竞争酶联免疫吸附测定法对获得的抗体进行表征，结果显示其具有出色的特异性和灵敏度，半数抑制浓度（IC50）为3.2 ppb，并且与大多数相关物种和化合物无交叉反应。鉴于硝基呋喃经常被非法用于通过饮用水喂养动物，我们测定了添加该药物的水中呋喃妥因的残留量。批间回收率在88 - 103%范围内，批内回收率在90 - 103%范围内。批间变异系数（CV）在3.1 - 11.4%范围内，批内变异系数在2.7 - 6.2%范围内。检测限确定为0.2 ppb。本研究中开发的免疫测定法适用于作为一种筛查方法来检测动物饮用水中呋喃妥因的残留量。
