将成体人类神经干细胞重编程为诱导多能干细胞。
Reprogramming of adult human neural stem cells into induced pluripotent stem cells.
机构信息
Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, China.
出版信息
Chin Med J (Engl). 2013 Mar;126(6):1138-43.
BACKGROUND
Since an effective method for generating induced pluripotent stem cells (iPSCs) from human neural stem cells (hNSCs) can offer us a promising tool for studying brain diseases, here we reported direct reprogramming of adult hNSCs into iPSCs by retroviral transduction of four defined factors.
METHODS
NSCs were successfully isolated and cultured from the hippocampus tissue of epilepsy patients. When combined with four factors (OCT3/4, SOX2, KLF4, and c-MYC), iPSCs colonies were successfully obtained.
RESULTS
Morphological characterization and specific genetic expression confirmed that these hNSCs-derived iPSCs showed embryonic stem cells-like properties, which include the ability to differentiate into all three germ layers both in vitro and in vivo.
CONCLUSION
Our method would be useful for generating human iPSCs from NSCs and provide an important tool for studying neurological diseases.
背景
由于从人神经干细胞（hNSC）中生成诱导多能干细胞（iPSC）的有效方法为我们研究脑部疾病提供了一种很有前景的工具，因此我们在此报道了通过逆转录病毒转导四种确定的因子将成体 hNSC 直接重编程为 iPSC。
方法
我们成功地从癫痫患者的海马组织中分离和培养了 NSC。当与四种因子（OCT3/4、SOX2、KLF4 和 c-MYC）结合时，成功获得了 iPSC 集落。
结果
形态学特征和特定的基因表达证实，这些 hNSC 来源的 iPSC 表现出胚胎干细胞样特性，包括在体外和体内分化为三个胚层的能力。
结论
我们的方法将有助于从 NSC 生成人 iPSC，并为研究神经疾病提供重要工具。
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