Biomedical Sciences Research Complex, University of St. Andrews, St. Andrews, Fife KY16 9ST, Scotland, United Kingdom.
J Proteomics. 2013 May 27;83:99-118. doi: 10.1016/j.jprot.2013.02.029. Epub 2013 Mar 16.
Brevibacterium linens DSM 20158 is an industrially important actinobacterium which is well-known for the production of amino acids and enzymes. However, as this strain has an unsequenced genome, there is no detailed information regarding its proteome although another strain of this microbe, BL2, has a shotgun genome sequence. However, this still does not cover the entire scope of its proteome. The present study is carried out by first identifying proteins by homology matches using the Mascot search algorithm followed by an advanced approach using de novo sequencing and MS BLAST to expand the B. linens proteome. The proteins identified in the secretome and cellular portion appear to be involved in various metabolic and physiological processes of this unsequenced organism. This study will help to enhance the usability of this strain of B. linens in different areas of research in the future rather than mainly in the food industries.
The present study describes the construction of the first detailed proteomic reference map of B. linens DSM 20158 with unsequenced genome by comparative proteome research analysis. This opens new horizons in proteomics to understand the role of proteins involved in the metabolism and physiology of other organisms with unsequenced genomes.
林奈短杆菌 DSM 20158 是一种具有工业重要性的放线菌，以生产氨基酸和酶而闻名。然而，由于该菌株的基因组尚未测序，尽管该微生物的另一个菌株 BL2 具有鸟枪法基因组序列，但关于其蛋白质组没有详细信息。然而，这仍然没有涵盖其蛋白质组的全部范围。本研究首先通过使用 Mascot 搜索算法进行同源匹配鉴定蛋白质，然后使用从头测序和 MS BLAST 进行高级方法扩展林奈短杆菌的蛋白质组。在分泌体和细胞部分鉴定出的蛋白质似乎参与了这个未测序生物体的各种代谢和生理过程。这项研究将有助于提高该林奈短杆菌菌株在未来不同研究领域的可用性，而不仅仅是在食品工业中。
本研究通过比较蛋白质组学研究分析，描述了第一个具有未测序基因组的林奈短杆菌 DSM 20158 的详细蛋白质组参考图谱的构建。这为理解涉及其他未测序基因组生物体代谢和生理的蛋白质的作用开辟了蛋白质组学的新视野。
