College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Key Lab for Animal Biotechnology of Agriculture Ministry, Northwest A&F University, Yangling, Shaanxi, China.
Cell Biochem Funct. 2013 Jun;31(4):281-8. doi: 10.1002/cbf.2970. Epub 2013 Mar 19.
Reproduction is required for the survival of all mammalian animals. Spermatogenesis is an essential and complex developmental process that ultimately results in production of haploid spermatozoa. Recent studies demonstrated that Boule and stimulated by retinoic acid 8 (Stra8) played important roles in initiation meiosis in male germ cells. miR-34c is indispensable in the late steps of spermatogenesis; remarkably, the main function of miR-34c is to reduce cell proliferation potentiality and promote cellular apoptosis. The objectives of this study were to investigate the expression patterns of Boule, Stra8, P53 and miR-34c in dairy goat testis and their relationship in male germ line stem cells (mGSCs). The results first revealed the expression patterns of Boule, Stra8, P53 and miR-34c in 30 dpp, 90 dpp and adult testes of dairy goats. The expression levels of Boule, Stra8, P53 and miR-34c in adult dairy goat testes were significantly higher than that of 30 dpp. Overexpression of Boule and Stra8 promoted the expression of miR-34c in dairy goat mGSCs. In our previous study, we showed that miR-34c was P53 dependent in mGSCs. These results have shown that the up-regulation of miR-34c was not due to P53 protein activation but which might be caused by the up-regulation of Boule and Stra8 promoting the advance of meiosis. In addition, we found retinoic acid would decrease the expression of P53 and miR-34c, however, did not change the expression of c-Myc greatly. It suggested that the function of driving differentiation of dairy goat mGSCs by retinoic acid might not be caused by P53.
所有哺乳动物的生存都需要繁殖。精子发生是一个必不可少且复杂的发育过程，最终导致产生单倍体精子。最近的研究表明，Boule 和视黄酸诱导 8（Stra8）在雄性生殖细胞的减数分裂起始中发挥重要作用。miR-34c 在精子发生的后期步骤中是不可或缺的；值得注意的是，miR-34c 的主要功能是降低细胞增殖潜力并促进细胞凋亡。本研究的目的是研究 Boule、Stra8、P53 和 miR-34c 在奶山羊睾丸中的表达模式及其在雄性生殖干细胞（mGSCs）中的关系。结果首次揭示了 Boule、Stra8、P53 和 miR-34c 在 30 dpp、90 dpp 和成年奶山羊睾丸中的表达模式。成年奶山羊睾丸中 Boule、Stra8、P53 和 miR-34c 的表达水平明显高于 30 dpp。Boule 和 Stra8 的过表达促进了 miR-34c 在奶山羊 mGSCs 中的表达。在我们之前的研究中，我们表明 miR-34c 在 mGSCs 中依赖于 P53。这些结果表明，miR-34c 的上调不是由于 P53 蛋白的激活，而是由于 Boule 和 Stra8 的上调促进了减数分裂的提前。此外，我们发现视黄酸会降低 P53 和 miR-34c 的表达，但对 c-Myc 的表达影响不大。这表明视黄酸驱动奶山羊 mGSCs 分化的功能可能不是由 P53 引起的。
