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骨髓间充质干细胞减少顺铂和围绝经期诱导的大鼠颗粒细胞凋亡。

BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopause.

作者信息

Guo Jun-Qi, Gao Xia, Lin Zhi-Jie, Wu Wei-Zhen, Huang Liang-Hu, Dong Hui-Yue, Chen Jin, Lu Jun, Fu Yun-Fen, Wang Jin, Ma Yu-Jie, Chen Xiao-Wen, Wu Zhi-Xian, He Fu-Qiang, Yang Shun-Liang, Liao Lian-Ming, Zheng Feng, Tan Jian-Ming

出版信息

BMC Cell Biol. 2013 Mar 19;14:18. doi: 10.1186/1471-2121-14-18.

DOI:10.1186/1471-2121-14-18
PMID:23510080
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3640998/
Abstract

BACKGROUND

The objective of this study was to evaluate the effect of bone marrow mesenchymal stem cells (BMSCs) on the apoptosis of granulosa cells (GCs) in rats.

RESULTS

Cisplatin increased GC apoptosis from 0.59% to 13.04% in the control and cisplatin treatment groups, respectively, which was significantly reduced upon co-culture with BMSCs to 4.84%. Cisplatin treatment increased p21 and bax and decreased c-myc mRNA expression, which was reversed upon co-culture with BMSCs. As compared to young rats, increased apoptosis was observed in the perimenopausal rats (P < 0.001). After 3 months, the apoptosis rate in the BMSC group was significantly lower than that of the control group (P = 0.007).

CONCLUSIONS

BMSC therapy may protect against GC apoptosis induced by cisplatin and perimenopause. Further studies are necessary to evaluate therapeutic efficacy of BMSCs.

摘要

背景

本研究的目的是评估骨髓间充质干细胞（BMSCs）对大鼠颗粒细胞（GCs）凋亡的影响。

结果

在对照组和顺铂治疗组中，顺铂分别使GC凋亡率从0.59%提高到13.04%，与BMSCs共培养后，凋亡率显著降低至4.84%。顺铂治疗增加了p21和bax的表达，并降低了c-myc mRNA的表达，与BMSCs共培养后这些变化发生了逆转。与年轻大鼠相比，围绝经期大鼠的凋亡增加（P < 0.001）。3个月后，BMSC组的凋亡率显著低于对照组（P = 0.007）。

结论

BMSC治疗可能预防顺铂和围绝经期诱导的GC凋亡。有必要进一步研究以评估BMSCs的治疗效果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f67/3640998/ae78c8513724/1471-2121-14-18-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f67/3640998/c10ae65c5abf/1471-2121-14-18-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f67/3640998/ae8e54316ec0/1471-2121-14-18-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f67/3640998/5aa78e60527a/1471-2121-14-18-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f67/3640998/e2f8a20b8739/1471-2121-14-18-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f67/3640998/ae78c8513724/1471-2121-14-18-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f67/3640998/c10ae65c5abf/1471-2121-14-18-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f67/3640998/ae8e54316ec0/1471-2121-14-18-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f67/3640998/5aa78e60527a/1471-2121-14-18-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f67/3640998/e2f8a20b8739/1471-2121-14-18-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f67/3640998/ae78c8513724/1471-2121-14-18-5.jpg

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