Faculty of Engineering and Science, University of Greenwich, Medway Campus, Central Avenue, Chatham Maritime, Kent ME4 4TB, UK.
Macromol Biosci. 2013 May;13(5):641-9. doi: 10.1002/mabi.201200462. Epub 2013 Mar 19.
A series of nanoparticles is prepared via layer-by-layer assembly of oppositely charged, synthetic biocompatible polyamidoamine polymers as potential carriers. Particle size, surface charge and internal chain mobility are quantified as a function of the polymer type and number of layers. The effect of addition of surfactant is examined to simulate the effects of nanoparticle dissolution. The cyctotoxicity of these particles (in epithelia and murine cell lines) are orders of magnitude lower than polyethyleneimine controls. Stable nanoparticles may be prepared from mixtures of strongly, oppositely charged polymers, but less successfully from weakly charged polymers, and, given their acceptable toxicity characteristics, such modularly designed constructs show promise for drug and gene delivery.
通过层层组装带相反电荷的合成生物相容的聚酰胺胺聚合物来制备一系列纳米颗粒，作为潜在的载体。作为聚合物类型和层数的函数，对颗粒大小、表面电荷和内部链流动性进行了量化。研究了添加表面活性剂的效果，以模拟纳米颗粒溶解的效果。这些颗粒（在上皮细胞和鼠细胞系中）的细胞毒性比聚乙烯亚胺对照物低几个数量级。可以从强、带相反电荷的聚合物混合物中制备稳定的纳米颗粒，但从弱电荷聚合物中则不太成功，并且，鉴于其可接受的毒性特征，这种模块化设计的构建体显示出在药物和基因传递方面的应用前景。
