Nagasaka A, Yoshida S, Ohyama T, Masunaga R, Kato S, Suzuki M, Itoh M, Kawabe T
Department of Internal Medicine, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
Horm Metab Res. 1990 Apr;22(4):229-31. doi: 10.1055/s-2007-1004890.
Isoelectric focusing studies indicated that three peaks of DNA polymerase beta (beta-enzyme) activity were focused at isoelectric point of 7.1, 8.2 and 9.1 in testes of sham-operated rats, whereas in testes of hypophysectomized rats only two peaks of the beta-enzyme activity appeared at pH 7.1 and 9.1 and the peak at pH 8.2 was reconstituted by the treatment of gonadotropins and testosterone. In the brain hypophysectomy did not affect the activities of isoelectrically different beta-enzymes. These facts imply that under in vivo conditions the beta-enzymes in testes focused at different isoelectric points are modified with unknown factors and the beta-enzyme focused at pH 8.2 is affected by pituitary hormones.
等电聚焦研究表明，在假手术大鼠的睾丸中，DNA聚合酶β（β-酶）活性的三个峰聚焦在等电点7.1、8.2和9.1处，而在垂体切除大鼠的睾丸中，β-酶活性仅在pH 7.1和9.1处出现两个峰，pH 8.2处的峰通过促性腺激素和睾酮处理得以恢复。在大脑中，垂体切除并不影响等电点不同的β-酶的活性。这些事实表明，在体内条件下，睾丸中聚焦于不同等电点的β-酶会被未知因素修饰，而聚焦于pH 8.2的β-酶受垂体激素影响。
