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Langmuir. 2013 Apr 16;29(15):4857-65. doi: 10.1021/la4002384. Epub 2013 Apr 5.
Oleic acid (OA) is well-known to affect the function of the skin barrier. In this study, the molecular interactions between OA and model stratum corneum (SC) lipids consisting of ceramide, cholesterol, and palmitic acid (PA) were investigated with Langmuir monolayer and associated techniques. Mixtures with different OA/SC lipid compositions were spread at the air/water interface, and the phase behavior was tracked with surface pressure-molecular area (π-A) isotherms. With increasing OA levels in the monolayer, the films became more fluid and more compressible. The thermodynamic parameters derived from π-A isotherms indicated that there are preferential interactions between OA and SC lipids and that films of their mixtures were thermodynamically stable. The domain structure and lipid conformational order of the monolayers were studied through Brewster angle microscopy (BAM) and infrared reflection absorption spectroscopy (IRRAS), respectively. Results indicate that lower concentrations of OA preferentially mix with and disorder the ceramide-enriched domains, followed by perturbation of the PA-enriched domains and disruption of SC lipid domain separation at higher OA levels.
油酸（OA）是众所周知的影响皮肤屏障功能。在这项研究中，油酸与由神经酰胺、胆固醇和棕榈酸（PA）组成的模型角质层（SC）脂质之间的分子相互作用通过 Langmuir 单层和相关技术进行了研究。用不同的 OA/SC 脂质组成的混合物在空气/水界面上展开，并通过表面压力-分子面积（π-A）等温线跟踪相行为。随着单层中 OA 水平的增加，膜变得更加流体和可压缩。从π-A 等温线上得出的热力学参数表明，OA 和 SC 脂质之间存在优先相互作用，并且它们的混合物膜在热力学上是稳定的。通过布鲁斯特角显微镜（BAM）和红外反射吸收光谱（IRRAS）分别研究了单层的域结构和脂质构象有序性。结果表明，较低浓度的 OA 优先与富含神经酰胺的区域混合并使其无序，然后在较高浓度的 OA 下扰乱富含 PA 的区域并破坏 SC 脂质的区域分离。
