Muto Mitsuru, Kaji Tatsuru, Mukai Motoi, Nakame Kazuhiko, Yoshioka Takako, Tanimoto Akihide, Matsufuji Hiroshi
Department of Pediatric Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 8908520, Japan.
Peptides. 2013 May;43:160-6. doi: 10.1016/j.peptides.2013.03.006. Epub 2013 Mar 18.
Children with short bowel syndrome face life-threatening complications. Therefore, there is an urgent need for a new therapy to induce effective adaptation of the remnant intestine. Adaptation occurs only during feeding. We focused on preprandial acyl ghrelin and des-acyl ghrelin, and postprandial glucagon-like peptide-2 (GLP-2), which are known to have active orexigenic and trophic actions. This study aims to clarify the secretion trends of these hormones after massive small bowel resection and to obtain basic data for developing a new treatment. Sixty-three growing male rats were used: 3 were designated as controls receiving no operation and 60 were randomized into the 80% small bowel resection (80% SBR) group and the transection and re-anastomosis group. Changes in body weight, food intake, and remnant intestine morphology were also assessed for 15 days after the operation. Acyl ghrelin and des-acyl ghrelin levels increased immediately, equivalently in both operation groups (P=0.09 and 0.70). Interestingly, in 80% SBR animals, des-acyl ghrelin peaked on day 1 and acyl ghrelin peaked on day 4 (P=0.0007 and P=0.049 vs controls). GLP-2 secretion was obvious in 80% SBR animals (P=2.25×10(-6)), which increased immediately and peaked on day 4 (P=0.009 vs. controls). Body weight and food intake in 80% SBR animals recovered to preoperative levels on day 4. Morphological adaptations were evident after day 4. Our results may suggest a management strategy to reinforce these physiological hormone secretion patterns in developing a new therapy for short bowel syndrome.
短肠综合征患儿面临危及生命的并发症。因此，迫切需要一种新的疗法来促使残余肠道有效适应。适应仅在进食期间发生。我们关注餐前的酰基胃饥饿素和去酰基胃饥饿素，以及餐后的胰高血糖素样肽-2（GLP-2），已知它们具有活跃的促食欲和营养作用。本研究旨在阐明大量小肠切除术后这些激素的分泌趋势，并为开发新的治疗方法获取基础数据。使用了63只生长中的雄性大鼠：3只作为未接受手术的对照组，60只随机分为80%小肠切除（80% SBR）组和横断再吻合组。术后15天还评估了体重、食物摄入量和残余肠道形态的变化。两个手术组的酰基胃饥饿素和去酰基胃饥饿素水平均立即升高，且升高程度相当（P = 0.09和0.70）。有趣的是，在80% SBR动物中，去酰基胃饥饿素在第1天达到峰值，酰基胃饥饿素在第4天达到峰值（与对照组相比，P = 0.0007和P = 0.049）。GLP-2分泌在80% SBR动物中很明显（P = 2.25×10(-6)），其立即升高并在第4天达到峰值（与对照组相比，P = 0.009）。80% SBR动物的体重和食物摄入量在第4天恢复到术前水平。术后第4天形态学适应明显。我们的结果可能提示在开发短肠综合征新疗法时强化这些生理激素分泌模式的管理策略。
