Molecular Mutagenesis & DNA Repair Unit, IRCCS Azienda Ospedaliera Universitaria San Martino - IST Istituto Nazionale Ricerca Cancro, Largo Rosanna Benzi n. 10, 16132 Genova, Italy.
Curr Med Chem. 2013;20(26):3272-99. doi: 10.2174/0929867311320260011.
To study novel treatments for high grade gliomas (WHO grade III and IV) we need animal models of those disorders. Orthotopic tumors in mouse or rats seem at present the most reliable in vivo glioma model in order to develop specific therapies. The orthotopic tumor characteristics should yet closely mimic the human glioma features (in particular infiltrating growth and neovascularization). In this regard, glioma cell lines with stem properties (glioma stem cells - GSC) may fulfill at best those needs. Orthotopic gliomas developed from some widely used, established non-stem cell lines showing poor infiltrating capacity are not suitable. Therapeutic and diagnostic procedures recently developed using orthotopic rodent glioma tumors along their potentialities and pitfalls are analyzed.
为了研究高级别脑胶质瘤（WHO 分级 III 和 IV）的新疗法，我们需要这些疾病的动物模型。目前，在小鼠或大鼠中建立的原位肿瘤似乎是最可靠的体内脑胶质瘤模型，以便开发特定的治疗方法。原位肿瘤的特征应该尽可能地模拟人类脑胶质瘤的特征（特别是浸润性生长和新生血管形成）。在这方面，具有干细胞特性的胶质瘤细胞系（胶质瘤干细胞-GSC）可能最好地满足这些需求。由一些广泛使用的、具有低浸润能力的已建立的非干细胞系发展而来的原位脑胶质瘤并不适合。分析了使用原位啮齿动物脑胶质瘤肿瘤以及它们的潜力和陷阱最近开发的治疗和诊断程序。
