Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland.
Reprod Biol. 2013 Mar;13(1):15-23. doi: 10.1016/j.repbio.2013.01.170. Epub 2013 Jan 22.
Progesterone (P4) is involved in the regulation of essential reproductive functions affecting the target cells through both nuclear progesterone receptors (PGRs) and membrane progesterone receptors. The aim of this study was to determine the mRNA and protein expression for PGRMC1, PGRMC2, SERBP1 and PGR within the bovine endometrium during the estrous cycle and the first trimester of pregnancy. There were no changes in PGRMC1 and PGRMC2 mRNA and protein expression during the estrous cycle, however, mRNA levels of PGRMC1 and PGRMC2 were increased (P<0.001) in pregnant animals. SERBP1 mRNA expression was increased (P<0.05), while the level of this protein was decreased (P<0.05) on days 11-16 of the estrous cycle. The expression of PGR mRNA was higher (P<0.01) on days 17-20 compared to days 6-10 and 11-16 of the estrous cycle and pregnancy. PGR-A and PGR-B protein levels were elevated on days 1-5 and 17-20 of the estrous cycle as compared to other stages of the cycle and during pregnancy. In conclusion, our results indicate that P4 may influence endometrial cells through both genomic and nongenomic way. This mechanism may contribute to the regulation of the estrous cycle and provide protection during pregnancy.
孕激素（P4）通过核孕激素受体（PGRs）和膜孕激素受体参与调节重要的生殖功能，作用于靶细胞。本研究旨在确定牛子宫内膜在发情周期和妊娠早期的 PGRMC1、PGRMC2、SERBP1 和 PGR 的 mRNA 和蛋白表达。在发情周期中，PGRMC1 和 PGRMC2 的 mRNA 和蛋白表达没有变化，然而，怀孕动物的 PGRMC1 和 PGRMC2 的 mRNA 水平增加（P<0.001）。SERBP1 mRNA 表达增加（P<0.05），而这种蛋白质的水平在发情周期的 11-16 天下降（P<0.05）。PGR mRNA 的表达在发情周期的 17-20 天高于 6-10 天和 11-16 天（P<0.01），并且在怀孕时也高于发情周期的其他阶段。PGR-A 和 PGR-B 蛋白水平在发情周期的 1-5 天和 17-20 天升高，与周期的其他阶段和怀孕期间相比。总之，我们的结果表明，P4 可能通过基因组和非基因组途径影响子宫内膜细胞。这种机制可能有助于调节发情周期，并在怀孕期间提供保护。
