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Preparation of the nematode-trapping fungus, Arthrobotrys oligospora, for scanning electron microscopy by freeze substitution.

作者信息

Wharton D A, Murray D S

机构信息

Department of Zoology, University of Otago, Dunedin, New Zealand.

出版信息

J Microsc. 1990 Apr;158(Pt 1):81-5. doi: 10.1111/j.1365-2818.1990.tb02979.x.

DOI:10.1111/j.1365-2818.1990.tb02979.x
PMID:2352273
Abstract

A freeze-substitution technique for preparing fungal specimens for scanning electron microscopy is described. This involves cryofixation in liquid nitrogen, freeze substitution in methanol at -20 degrees C and critical-point drying. The trapping complexes and conidiophores of the nematophagous fungus Arthrobotrys oligospora are well preserved and retain their normal three-dimensional arrangement.

摘要

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