Institute of Biochemistry, Department of Biotechnology and Enzyme Catalysis, University of Greifswald, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany.
Curr Opin Chem Biol. 2013 Apr;17(2):215-20. doi: 10.1016/j.cbpa.2013.02.022. Epub 2013 Mar 21.
Protein engineering is the most important method to overcome the limitations of natural enzymes as biocatalysts. The past few years have seen a tremendous increase in novel concepts to facilitate the design of mutant libraries for focused directed evolution mostly guided by advanced bioinformatic tools. In addition, advanced high-throughput methods were developed using, for example, FACS analysis or microfluidic systems. These achievements significantly facilitate the tailor-made design of enzymes to make them suitable for industrial applications.
蛋白质工程是克服天然酶作为生物催化剂的局限性的最重要方法。在过去的几年中，出现了许多新概念，这些概念借助先进的生物信息学工具，有助于设计针对定向进化的突变文库。此外，还开发了先进的高通量方法，例如使用 FACS 分析或微流控系统。这些成就极大地促进了酶的定制设计，使其适用于工业应用。
