Dipartimento di Scienze Biomediche, Università di Cagliari, Cittadella Universitaria, 09042 Monserrato (CA), Italy.
Phytochemistry. 2013 Jun;90:16-24. doi: 10.1016/j.phytochem.2013.02.019. Epub 2013 Mar 21.
Ferula communis (L.), a plant belonging to Apiaceae, is widely present in Sardinia, Italy. Currently, interest in F. communis focuses on the presence of two chemotypes in the wild. One chemotype is poisonous to animals, whereas the other chemotype is non-poisonous. Polyphenol oxidase (PPO) has been extracted and partially purified from the two chemotypes of F. communis. The biochemical characterization of the enzymes showed significant differences. In particular, while the two PPOs were not able to use 6- and 7-hydroxycoumarin as substrates, they showed distinct specificity for 6,7- and 7,8-dihydroxycoumarin. Significant differences in the enzyme behavior towards common PPO inhibitors were also observed. In addition, activation energy and activation energy for denaturation were determined, showing significant differences between FP-PPO and FNP-PPO, particularly for denaturation kinetics. The possible roles of the two PPOs in determining differences in composition and toxicity of the two F. communis chemotypes are also discussed.
阿魏属（L.），伞形科植物，在意大利撒丁岛广泛存在。目前，人们对 F. communis 的兴趣集中在其野生状态下存在两种化学型上。一种化学型对动物有毒，而另一种化学型则无毒。多酚氧化酶（PPO）已从 F. communis 的两种化学型中提取并部分纯化。对酶的生化特性进行了研究，结果表明存在显著差异。特别是，虽然两种 PPO 都不能使用 6-和 7-羟基香豆素作为底物，但它们对 6,7-和 7,8-二羟基香豆素表现出明显的特异性。对常见 PPO 抑制剂的酶行为也观察到显著差异。此外，还测定了酶的活化能和变性活化能，FP-PPO 和 FNP-PPO 之间存在显著差异，特别是在变性动力学方面。还讨论了两种 PPO 在决定两种 F. communis 化学型组成和毒性差异方面的可能作用。
