Consiglio per la Ricerca e la Sperimentazione in Agricoltura (CRA)-Centro di Ricerca per la Frutticoltura, Rome, Italy.
Nat Genet. 2013 May;45(5):487-94. doi: 10.1038/ng.2586. Epub 2013 Mar 24.
Rosaceae is the most important fruit-producing clade, and its key commercially relevant genera (Fragaria, Rosa, Rubus and Prunus) show broadly diverse growth habits, fruit types and compact diploid genomes. Peach, a diploid Prunus species, is one of the best genetically characterized deciduous trees. Here we describe the high-quality genome sequence of peach obtained from a completely homozygous genotype. We obtained a complete chromosome-scale assembly using Sanger whole-genome shotgun methods. We predicted 27,852 protein-coding genes, as well as noncoding RNAs. We investigated the path of peach domestication through whole-genome resequencing of 14 Prunus accessions. The analyses suggest major genetic bottlenecks that have substantially shaped peach genome diversity. Furthermore, comparative analyses showed that peach has not undergone recent whole-genome duplication, and even though the ancestral triplicated blocks in peach are fragmentary compared to those in grape, all seven paleosets of paralogs from the putative paleoancestor are detectable.
蔷薇科是最重要的水果生产类群，其主要商业相关属（草莓属、蔷薇属、悬钩子属和李属）表现出广泛的不同生长习性、果实类型和紧凑的二倍体基因组。桃是李属的一个二倍体种，是落叶树中遗传特征最好的树种之一。在这里，我们描述了来自完全纯合基因型的桃的高质量基因组序列。我们使用桑格全长鸟枪法获得了一个完整的染色体规模的组装。我们预测了 27852 个编码蛋白质的基因和非编码 RNA。我们通过对 14 个李属植物的全基因组重测序研究了桃的驯化途径。分析表明，主要的遗传瓶颈对桃基因组多样性产生了重大影响。此外，比较分析表明，桃没有经历过近期的全基因组复制，尽管桃的祖先三倍体块与葡萄相比是零碎的，但来自假定的古祖先的 7 个古近系的旁系同源物都可以检测到。
