Department of Pediatric Surgery, Inönü University Medical School, Malatya, Turkey.
Clinics (Sao Paulo). 2013;68(2):239-44. doi: 10.6061/clinics/2013(02)oa19.
In addition to its roles in the stimulation of growth hormone secretion and the regulation of appetite and metabolism, ghrelin exerts immunomodulatory, anti-inflammatory and antioxidant actions in several organ systems. In this study, we investigated the effects of ghrelin on the healing of experimental colonic anastomoses.
Wistar rats were randomly divided into two groups (n = 10 in each). A segment of colon was excised, and an end-to-end anastomosis was performed in the distal colon. The Ghrelin Group received 10 ng/kg/day IP ghrelin for seven days postoperatively, whereas the Control Group received an identical volume of saline. On the seventh postoperative day, the anastomotic bursting pressures and hydroxyproline levels were measured, and adhesion formation around the anastomoses was examined. Histopathological analyses were performed to evaluate inflammatory cell infiltration, fibroblast infiltration, collagen density and neovascularization.
In the Ghrelin Group, the bursting pressure and hydroxyproline levels were significantly higher than in the Control Group. The adhesion formation scores were lower in the Ghrelin Group than in the Control Group. Although the inflammatory cell infiltration was diminished in the Ghrelin Group, the degrees of fibroblast infiltration, collagen density and neovascularization were not significantly different between the groups.
Our results indicate that ghrelin improves the healing of colonic anastomoses in rats.
除了在刺激生长激素分泌和调节食欲和代谢方面发挥作用外，ghrelin 在多个器官系统中还具有免疫调节、抗炎和抗氧化作用。在这项研究中，我们研究了 ghrelin 对实验性结肠吻合口愈合的影响。
将 Wistar 大鼠随机分为两组（每组 10 只）。切除一段结肠，并在远端结肠进行端端吻合。Ghrelin 组在术后 7 天内每天接受 10ng/kg/天的 IP ghrelin，而对照组则接受相同体积的生理盐水。在术后第 7 天，测量吻合口爆裂压和羟脯氨酸水平，并检查吻合口周围的粘连形成情况。进行组织病理学分析以评估炎症细胞浸润、成纤维细胞浸润、胶原密度和新生血管形成。
Ghrelin 组的爆裂压和羟脯氨酸水平明显高于对照组。Ghrelin 组的粘连形成评分低于对照组。尽管 Ghrelin 组的炎症细胞浸润减少，但两组之间的成纤维细胞浸润、胶原密度和新生血管形成程度没有显著差异。
我们的结果表明，ghrelin 可改善大鼠结肠吻合口的愈合。
