Rodrigo G C, Chapman R A
Department of Physiology, School of Veterinary Science, Bristol, UK.
Pflugers Arch. 1990 Apr;416(1-2):196-200. doi: 10.1007/BF00370242.
A method for the manufacture of ion-sensitive micro-electrodes, which can be readily used with small single cells, is described in detail. A glass pipette with a tip size of 1 micron, essentially similar to those used as suction electrodes in whole-cell recording, when silanized and with its tip filled with a suitable ion-sensitive resin, produces an ion-sensitive electrode with fast electrical and chemical response times. These electrodes can be applied to the cell membrane of isolated myocytes and penetration achieved without cell damage, by the application of suction. For the estimation of intracellular ionic activities they can be used in conjunction with a separate conventional KCl-filled micro-electrode or a suction voltage electrode. The technique is illustrated by the measurement of intracellular Na+, Ca2+ and pH. It is possible that these electrodes can also be used to measure local changes in ionic activity in restricted areas.
详细描述了一种制造离子敏感微电极的方法，该微电极可方便地用于小型单细胞。一种尖端尺寸为1微米的玻璃吸管，本质上类似于全细胞记录中用作吸电极的吸管，经硅烷化处理并在其尖端填充合适的离子敏感树脂后，可制成具有快速电响应和化学响应时间的离子敏感电极。通过施加吸力，这些电极可应用于分离的心肌细胞的细胞膜，实现穿透且不会对细胞造成损伤。为了估计细胞内离子活性，它们可与单独的传统填充KCl的微电极或吸电压电极结合使用。通过测量细胞内Na+、Ca2+和pH值来说明该技术。这些电极也有可能用于测量受限区域内离子活性的局部变化。
