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通过聚合物纳米纤维嵌入微芯片进行高纯度前列腺循环肿瘤细胞分离以用于全外显子组测序

High-purity prostate circulating tumor cell isolation by a polymer nanofiber-embedded microchip for whole exome sequencing.

作者信息

Zhao Libo, Lu Yi-Tsung, Li Fuqiang, Wu Kui, Hou Shuang, Yu Juehua, Shen Qinglin, Wu Dongxia, Song Min, OuYang Wei-Han, Luo Zheng, Lee Tom, Fang Xiaohong, Shao Chen, Xu Xun, Garcia Mitch A, Chung Leland W K, Rettig Matthew, Tseng Hsian-Rong, Posadas Edwin M

机构信息

Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry, Chinese Academy of Science, Beiyi Street 2#, Zhongguancun, Beijing, 100190, P. R. China; Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), University of California at Los Angeles, 570 Westwood Plaza, Build 114, Los Angeles, California 90095-1770, USA.

出版信息

Adv Mater. 2013 Jun 4;25(21):2897-902. doi: 10.1002/adma.201205237. Epub 2013 Mar 26.

DOI:10.1002/adma.201205237
PMID:23529932
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3875622/
Abstract

Handpick single cancer cells: a modified NanoVelcro Chip is coupled with ArcturusXT laser capture microdissection (LCM) technology to enable the detection and isolation of single circulating tumor cells (CTCs) from patients with prostate cancer (PC). This new approach paves the way for conducting next-generation sequencing (NGS) on single CTCs.

摘要

挑选单个癌细胞

一种经过改良的纳米维可牢芯片与ArcturusXT激光捕获显微切割（LCM）技术相结合，能够从前列腺癌（PC）患者中检测和分离单个循环肿瘤细胞（CTC）。这种新方法为对单个CTC进行下一代测序（NGS）铺平了道路。

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