Auckland Cancer Society Research Centre, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand.
Nutr Cancer. 2013;65(3):355-66. doi: 10.1080/01635581.2013.760743.
Selenium (Se) supplementation was tested in a group of healthy men from Auckland, New Zealnd with selenized yeast (Selplex, 200 μg/day) as the supplementation mode. A set of biomarkers, including DNA damage levels and seleno-antioxidant enzyme levels, were evaluated at pre- and postsupplementation time points. Supplementation produced significant increases in serum Se levels, red blood cell (RBC) thioredoxin reductase (TR) activity and peroxide-induced DNA damage, when the mean baseline serum Se level was 110 ng/ml. Those with higher baseline serum Se levels gained less serum Se and showed a significant reduction of RBC glutathione peroxidase (GPx) activity by supplementation. The optimum benefits of supplementation on DNA stability are observed when the serum Se level reaches between >120 and <160 ng/ml. However, the most significant observation was that those with highest baseline DNA damage benefit the most from Se supplementation, whereas those having lower baseline DNA damage are disadvantaged. A dose of 200 μg/day selenized yeast was also shown to be a safer supplementation option compared to a similar dose of selenomethionine (SeMet). This study highlights the requirement for prestratification of a population by standing serum Se level and baseline DNA damage level, before any Se supplementation is carried out.
硒（Se）补充剂已在新西兰奥克兰的一组健康男性中进行了测试，补充方式为富硒酵母（Selplex，每天 200μg）。在补充前后的时间点评估了一组生物标志物，包括 DNA 损伤水平和硒抗氧化酶水平。当平均基线血清 Se 水平为 110ng/ml 时，补充后血清 Se 水平、红细胞（RBC）硫氧还蛋白还原酶（TR）活性和过氧化物诱导的 DNA 损伤显著增加。那些基线血清 Se 水平较高的人获得的血清 Se 较少，并且补充后 RBC 谷胱甘肽过氧化物酶（GPx）活性显著降低。当血清 Se 水平在 120-160ng/ml 之间时，补充对 DNA 稳定性的最佳益处最为明显。然而，最显著的观察结果是，那些基线 DNA 损伤最高的人从 Se 补充中获益最多，而那些基线 DNA 损伤较低的人则处于劣势。与类似剂量的硒蛋氨酸（SeMet）相比，每天 200μg 富硒酵母也被证明是一种更安全的补充选择。本研究强调，在进行任何 Se 补充之前，需要根据基线血清 Se 水平和基线 DNA 损伤水平对人群进行分层。
