Ito T, Kobayashi K
Biochim Biophys Acta. 1975 Jan 6;378(1):125-32. doi: 10.1016/0005-2787(75)90143-4.
Acridine orange was used as a probe to look into the physiological state of the yeast cell, particularly as regards the change in the properties of the membrane (which acts as a barrier against the incoming acridine orange) and the availability of binding sites for acridine orange in chromosomal DNA during growth. After acridine orange had been introduced into the cell, the genetic change at a specific locus with incubation time was measured photodynamically. A three-fold increase in the rate of penetration of acridine orange into the cell was observed, for instance, in going from the resting phase to the dividing phase. A five-fold increase was observed in the number of binding sites in chromosomal DNA under the same transition of the cell. These two parameters may be useful as a measure of the physiological changes in the cell. Some environmental factors such as pH and temperature were also demonstrated to affect the parameters.
吖啶橙被用作一种探针来研究酵母细胞的生理状态，特别是关于膜（作为阻止进入的吖啶橙的屏障）性质的变化以及在生长过程中染色体DNA上吖啶橙结合位点的可用性。在将吖啶橙引入细胞后，通过光动力学测量特定基因座处随孵育时间的遗传变化。例如，从静止期到分裂期，观察到吖啶橙进入细胞的渗透率增加了三倍。在细胞的相同转变下，观察到染色体DNA中结合位点的数量增加了五倍。这两个参数可作为细胞生理变化的一种度量。还证明了一些环境因素，如pH值和温度，会影响这些参数。
