Advanced Polymer & Nanomaterial Laboratory, Department of Chemical Sciences, Tezpur University, Tezpur-784028, Assam, India.
Biomed Mater. 2013 Jun;8(3):035003. doi: 10.1088/1748-6041/8/3/035003. Epub 2013 Mar 26.
The fabrication of a smart magnetically controllable bio-based polymeric nanocomposite (NC) has immense potential in the biomedical domain. In this context, magneto-thermoresponsive sunflower oil modified hyperbranched polyurethane (HBPU)/Fe3O4 NCs with different wt.% of magnetic nanoparticles (Fe3O4) were prepared by an in situ polymerization technique. Fourier-transform infrared, x-ray diffraction, vibrating sample magnetometer, scanning electron microscope, transmission electron microscope, thermal analysis and differential scanning calorimetric were used to analyze various physico-chemical structural attributes of the prepared NC. The results showed good interfacial interactions between HBPU and well-dispersed superparamagnetic Fe3O4, with an average diameter of 7.65 nm. The incorporation of Fe3O4 in HBPU significantly improved the thermo-mechanical properties along with the shape-memory behavior, antibacterial activity, biocompatibility as well as biodegradability in comparison to the pristine system. The cytocompatibility of the degraded products of the NC was also verified by in vitro hemolytic activity and MTT assay. In addition, the in vivo biocompatibility and non-immunological behavior, as tested in Wistar rats after subcutaneous implantation, show promising signs for the NC to be used as antibacterial biomaterial for biomedical device and implant applications.
制备智能磁控生物基聚合物纳米复合材料(NC)在生物医学领域具有巨大的潜力。在这种情况下,通过原位聚合技术制备了不同质量分数的磁性纳米粒子(Fe3O4)的磁热响应葵花油改性超支化聚氨酯(HBPU)/Fe3O4 NC。傅里叶变换红外光谱、X 射线衍射、振动样品磁强计、扫描电子显微镜、透射电子显微镜、热分析和差示扫描量热法用于分析所制备 NC 的各种物理化学结构特性。结果表明,HBPU 与分散良好的超顺磁 Fe3O4 之间具有良好的界面相互作用,平均粒径为 7.65nm。与原始体系相比,Fe3O4 的加入显著提高了 HBPU 的热机械性能以及形状记忆性能、抗菌活性、生物相容性和可生物降解性。通过体外溶血活性和 MTT 测定还验证了 NC 降解产物的细胞相容性。此外,在皮下植入 Wistar 大鼠后进行体内生物相容性和非免疫性测试,表明 NC 有望作为抗菌生物材料用于生物医学设备和植入物应用。