Moretti Massimo, Villarini Milena, Dominici Luca, Fatigoni Cristina, dell'Omo Marco, Elisei Emanuela, Muzi Giacomo, Monarca Silvano
Dipartimento di Specialità Medico-Chirurgiche e Sanità Pubblica (Sez. di Sanità Pubblica), Università degli Studi di Perugia, Perugia, Italy.
Ig Sanita Pubbl. 2013 Jan-Feb;69(1):55-77.
The present molecular epidemiology study was carried out to evaluate the genotoxic effects of occupational exposure to antineoplastic drugs (ANP). The study was conducted in 52 hospital workers involved in the preparation, handling or administration of ANP in a hospital in Perugia (central Italy) and in 52 non-exposed control subjects matched for age, gender and smoking habits to the exposed subjects. Both comet assay and the micronucleus test were used to evaluate genome damage in peripheral blood lymphocytes in study subjects. The extent of primary DNA damage, as evaluated by the comet assay, was significantly increased in exposed personnel with respect to matched controls. On the other hand, no significant differences in micronuclei frequency was observed between the two groups. Multivariate linear regression analysis showed an association between years of occupational exposure over 10 years and higher extent of primary DNA damage in the exposed group. The results of this study confirm that handling ANP without appropriate precautions carries a genotoxic risk for exposed healthcare workers. These results address the need for regular biological effect monitoring of staff occupationally-exposed to ANP.
本分子流行病学研究旨在评估职业接触抗肿瘤药物（ANP）的遗传毒性效应。该研究在意大利中部佩鲁贾一家医院的52名参与ANP制备、处理或给药的医院工作人员中进行，并在年龄、性别和吸烟习惯与暴露组匹配的52名未暴露对照受试者中开展。彗星试验和微核试验均用于评估研究对象外周血淋巴细胞中的基因组损伤。通过彗星试验评估，暴露人员的原发性DNA损伤程度相对于匹配对照显著增加。另一方面，两组之间微核频率未观察到显著差异。多变量线性回归分析显示，暴露组中职业暴露超过10年与原发性DNA损伤程度较高之间存在关联。本研究结果证实，在没有适当预防措施的情况下处理ANP会给暴露的医护人员带来遗传毒性风险。这些结果表明有必要对职业接触ANP的工作人员进行定期生物效应监测。
