体外成熟卵母细胞的极体碎片化与受精和胚胎发育受损有关。
Polar body fragmentation in IVM oocytes is associated with impaired fertilization and embryo development.
机构信息
Infertility Solutions, P. C., Allentown, PA 18103, USA.
出版信息
J Assist Reprod Genet. 2013 Jun;30(5):679-82. doi: 10.1007/s10815-013-9982-4. Epub 2013 Mar 27.
PURPOSE
The significance of finding a fragmented first polar body in an oocyte prepared for ICSI is controversial with most recent publications suggesting that it is not prognostic for oocyte fertilization or embryo development. Our purpose was to look at this question in the context of oocytes not stimulated for conventional IVF.
METHODS
Oocytes obtained for IVM and obtained from follicles at most 12 mm in diameter were evaluated for their polar body morphology soon after they entered metaphase II when they were denuded in preparation for ICSI. Records were evaluated retrospectively for the fertilization rate and the embryo growth rate (cell number) on each day of development for embryos with normal appearing polar bodies or fragmented polar bodies, but no other cytoplasmic dysmorphisms.
RESULTS
Oocytes with fragmented polar bodies were significantly less likely to fertilize than oocytes with normal appearing polar bodies (p < 0.0001). Embryos which developed from oocytes with fragmented polar bodies had significantly impaired growth compared to embryos that developed from oocytes with normal appearing polar bodies (p = 0.0328).
CONCLUSIONS
Fragmented polar bodies likely reflect cytoplasmic incompetence.
目的
在准备进行 ICSI 的卵母细胞中发现碎片化的第一极体的意义存在争议，大多数最新的出版物表明，它对卵母细胞受精或胚胎发育没有预后意义。我们的目的是在不进行常规 IVF 刺激的卵母细胞的背景下研究这个问题。
方法
在进入第二次减数分裂中期后不久，即准备进行 ICSI 时，对体外成熟（IVM）获得的卵母细胞和最大直径为 12 毫米的卵泡中获得的卵母细胞进行极体形态评估。回顾性评估正常外观极体或碎片化极体的卵母细胞的受精率和胚胎发育率（细胞数），但无其他细胞质形态异常。
结果
碎片化极体的卵母细胞受精的可能性明显低于正常外观极体的卵母细胞（p < 0.0001）。与正常外观极体的卵母细胞发育而来的胚胎相比，碎片化极体的卵母细胞发育而来的胚胎生长明显受损（p = 0.0328）。
结论
碎片化极体可能反映细胞质功能不全。
Zotero 插件