在快速、与血清兼容的免疫测定中，高效Förster 共振能量转移。
Highly efficient förster resonance energy transfer in a fast, serum-compatible immunoassay.
机构信息
Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Center for Biotechnology and Biomedicine, Universität Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany.
出版信息
Chembiochem. 2013 Apr 15;14(6):699-702. doi: 10.1002/cbic.201300073. Epub 2013 Mar 26.
Highly efficient FRET leads to important enhancements for homogeneous immunoassays. By using the novel phosphorescent dye EuLH and BHQ-10 as a donor-acceptor pair, the FRET efficiency increases to >99.5 %, leading to significantly improved signal-to-background ratio, precision and linear range. The phosphorescence detection enabled full compatibility to serum samples for this fast-responding immunoassay.
高效的 FRET 导致均相免疫分析的重要增强。通过使用新型磷光染料 EuLH 和 BHQ-10 作为供体-受体对，FRET 效率增加到>99.5%，导致信号背景比、精密度和线性范围显著提高。该磷光检测使这种快速响应免疫分析完全兼容血清样本。
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