Gorris Hans H, Resch-Genger Ute
Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040, Regensburg, Germany.
Federal Institute for Materials Research and Testing (BAM), Richard-Willstaetter-Str. 11, 12489, Berlin, Germany.
Anal Bioanal Chem. 2017 Oct;409(25):5875-5890. doi: 10.1007/s00216-017-0482-8. Epub 2017 Jul 7.
In Part II of this review series on lanthanide-doped photon-upconversion nanoparticles (UCNPs), we present and critically discuss the performance and suitability of UCNPs as background-free luminescent reporters in bioimaging and bioanalytical applications. The preparation of a biocompatible nanoparticle surface is an integral step for all life - science-related applications. UCNPs have found their way into a large number of diagnostic platforms, homogeneous and heterogeneous assay formats, and sensor applications. Many bioanalytical detection schemes involve Förster resonance energy transfer (FRET), which is still debated for UCNPs and needs to be much improved. The need for dedicated and standardized instruments as well as recent studies on the dissolution and potential toxicity of UCNPs are addressed. Finally we outline future trends and challenges in the field of upconversion. Graphical Abstract Both synthesis / spectroscopy as well bioanalytical applications of UCNPs are driven by the COST Action CM1403 "The European Upconversion Network".
在关于镧系元素掺杂的光子上转换纳米颗粒（UCNPs）的本系列综述的第二部分中，我们展示并批判性地讨论了UCNPs作为生物成像和生物分析应用中无背景发光报告分子的性能和适用性。制备生物相容性纳米颗粒表面是所有生命科学相关应用不可或缺的一步。UCNPs已进入大量诊断平台、均相和异相分析形式以及传感器应用中。许多生物分析检测方案涉及Förster共振能量转移（FRET），这在UCNPs方面仍存在争议且需要大幅改进。文中还讨论了对专用和标准化仪器的需求以及近期关于UCNPs溶解和潜在毒性的研究。最后，我们概述了上转换领域未来的趋势和挑战。图形摘要UCNPs的合成/光谱以及生物分析应用均由“欧洲上转换网络”COST行动CM1403推动。
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