Institute of Pathology, RWTH Aachen University Hospital, Aachen, Germany.
J Clin Pathol. 2013 Aug;66(8):711-4. doi: 10.1136/jclinpath-2013-201543. Epub 2013 Mar 26.
α-Naphthyl acetate esterase (ANAE) is one of the few enzymes that are histochemically detectable on formalin-fixed paraffin-embedded tissue. In bone marrow (BM) biopsies, ANAE staining highlights megakaryocytes. We investigated autopsy BM to determine whether ANAE staining intensity (SI) was associated with postmortem intervals (PMI, period between death and autopsy), and thus could allow the time of death of a patient to be deduced.
ANAE-stained BM slides of 74 forensic and pathology autopsies as well as 22 biopsies were histologically evaluated and their SIs semiquantitatively graded.
ANAE-SIs did not differ between men and women and slightly decreased with age. Biopsies had significantly higher ANAE-SIs than pathology cases. In autopsies, ANAE-SIs were not associated with PMI, except for cases with PMI ≥7 days which were consistently ANAE-negative.
ANAE-SIs in postmortem BM samples were independent of PMI. Thus, ANAE staining of BM megakaryocytes cannot serve as an indicator for time-since-death of a patient.
α-萘乙酸酯酶（ANAE）是少数几种可在福尔马林固定石蜡包埋组织中进行组织化学检测的酶之一。在骨髓（BM）活检中，ANAE 染色突出巨核细胞。我们研究了尸检 BM，以确定 ANAE 染色强度（SI）是否与死后间隔时间（PMI，从死亡到尸检的时间）相关，从而可以推断患者的死亡时间。
对 74 例法医和病理学尸检以及 22 例活检的 ANAE 染色 BM 幻灯片进行组织学评估，并对其 SI 进行半定量分级。
ANAE-SI 在男性和女性之间没有差异，并且随着年龄的增长略有下降。活检的 ANAE-SI 明显高于病理学病例。在尸检中，除了 PMI≥7 天的病例始终为 ANAE 阴性外，ANAE-SI 与 PMI 无关。
死后 BM 样本中的 ANAE-SI 与 PMI 无关。因此，BM 巨核细胞的 ANAE 染色不能作为患者死亡时间的指标。
