Sanamyan Marina F, Petlyakova Julia E, Sharipova Elnora A, Abdurakhmonov Ibrokhim Y
Cotton Genetics Laboratory, National University of Uzbekistan, Tashkent, Vuzgorodok 100174, Uzbekistan.
Genet Res Int. 2011;2011:273642. doi: 10.4061/2011/273642. Epub 2011 Mar 20.
The use of aneuploid lines significantly increases the effectiveness of molecular-genetic analysis and the development of superior quality breeding lines via substitutions by alien chromosomes. To date, however, a complete set of aneuploid series for each cotton chromosome is not available. Here, we present the development of a monosomic stock collection of cotton (Gossypium hirsutum L.) from Uzbekistan, including the origin of 92 primary monosomics, meiotic metaphase-I analysis, study of tetrads of microspores, pollen fertility, and monosomic transmission rates for some monosomic lines. We report desynaptic effects of some monosomes detected both in parental and daughter monosomics, a positive role of interchanges in translocation heterozygous monosomics due to selective advantages of gametes with deficiency and a simultaneous interchange, pollen fertility variation, and strong differences in transmission rates. This monosomic cotton collection, developed using single genome background, will be useful for future breeding, genetic, cytogenetic, and molecular-genetic investigations of the cotton genome.
非整倍体系的使用通过异源染色体替换显著提高了分子遗传学分析的效率以及优质育种系的培育。然而，迄今为止，尚未获得针对每条棉花染色体的完整非整倍体系列。在此，我们展示了来自乌兹别克斯坦的棉花（陆地棉）单体植株收集品系的培育过程，包括92个初级单体的起源、减数分裂中期I分析、小孢子四分体研究、花粉育性以及部分单体品系的单体传递率。我们报道了在亲本和子代单体中检测到的一些单体的去联会效应、由于具有缺失和同时易位的配子的选择优势，互换在易位杂合单体中的积极作用、花粉育性变异以及传递率的显著差异。这个使用单一基因组背景培育的棉花单体收集品系，将对棉花基因组未来的育种、遗传、细胞遗传和分子遗传学研究有用。
