Wei Can, Lai Yong-Qing, Li Xian-Xin, Ye Jiong-Xian
Department of Urology, The Peking University Shenzhen Hospital, Shenzhen, China.
Asian Pac J Cancer Prev. 2013;14(1):315-20. doi: 10.7314/apjcp.2013.14.1.315.
TGF-β-activated kinase-1 (TAK1) has been found to be over-expressed in a variety of solid malignancies and related to tumor growth. The aim of this study was to evaluate the expression level of TAK1 in clear cell renal cell carcinoma (ccRCC) and assess its value as a novel prognostic marker.
TAK1 mRNA was assessed in 51 paired ccRCC tissues and adjacent normal tissues (ADTs) by real-time PCR. Tissue TAK1 protein was also assessed in 91 ADTs and 177 samples of ccRCC immunohistochemically for evaluation of relationships with clinical characteristics.
RT-PCR showed that TAK1 RNA level was significantly higher in ccRCC tissues than in the paired ADTs and immunohistochemistry confirmed higher expression of TAK1 protein in ccRCC samples compared with ADTs. TAK1 protein expression in 177 ccRCC samples was significantly correlated with T stage, N classification, metastasis, recurrence and Fuhrman grade, but not age and gender. Patients with low TAK1 levels had a better survival outcome. TAK1 expression and N stage were independent prognosis factors for the overall survival of ccRCC patients.
Overexpression of TAK1 predicts a poor prognosis in patients with ccRCC, so that TAK1 may serve as a novel prognostic marker.
已发现转化生长因子-β激活激酶-1（TAK1）在多种实体恶性肿瘤中过度表达，并与肿瘤生长相关。本研究的目的是评估TAK1在透明细胞肾细胞癌（ccRCC）中的表达水平，并评估其作为一种新型预后标志物的价值。
通过实时PCR评估51对ccRCC组织和相邻正常组织（ADT）中的TAK1 mRNA。还通过免疫组织化学评估了91个ADT和177个ccRCC样本中的组织TAK1蛋白，以评估其与临床特征的关系。
逆转录-聚合酶链反应（RT-PCR）显示，ccRCC组织中的TAK1 RNA水平显著高于配对的ADT，免疫组织化学证实，与ADT相比，ccRCC样本中TAK1蛋白的表达更高。177个ccRCC样本中的TAK1蛋白表达与T分期、N分级、转移、复发和Fuhrman分级显著相关，但与年龄和性别无关。TAK1水平低的患者生存结果更好。TAK1表达和N分期是ccRCC患者总生存的独立预后因素。
TAK1的过表达预示ccRCC患者预后不良，因此TAK1可作为一种新型预后标志物。
