Department of Biomedical Engineering and Environmental Sciences, National Tsing-Hua University, Hsinchu, 30013, Taiwan.
Nanotechnology. 2013 Apr 26;24(16):165101. doi: 10.1088/0957-4484/24/16/165101. Epub 2013 Mar 27.
Although the unique optical properties of surface-modified quantum dots (QDs) have attracted wide interest in molecular biology and bioengineering, there are very few reports of their in vivo biodistribution, due to a lack of analytical techniques for characterizing the dynamic variation of QDs in living animals. In this study, we used an in vivo online monitoring system and a batch-wise elemental analytical method to investigate the biodistribution/extravasation of various surface-modified CdTeSe/ZnS (QDs) in rat liver. It is found that the surface modification dictated not only the blood retention profile but also the degree of extravasation and the clearance of extracellular QDs, making it an important variable for regulating the transfer and exchange process of QDs among three physiological compartments-bloodstream, extracellular space and Kupffer cells/hepatocytes.
虽然表面修饰的量子点（QDs）独特的光学性质在分子生物学和生物工程中引起了广泛的关注，但由于缺乏分析技术来描述活体内 QD 的动态变化，因此很少有关于它们在体内生物分布的报道。在这项研究中，我们使用了一种体内在线监测系统和一种分批元素分析方法来研究不同表面修饰的 CdTeSe/ZnS（QDs）在大鼠肝脏中的生物分布/外渗情况。结果发现，表面修饰不仅决定了血液保留曲线，还决定了外渗程度和细胞外 QD 的清除程度，因此成为调节 QD 在血流、细胞外空间和库普弗细胞/肝细胞这三个生理隔室之间转移和交换过程的重要变量。
