通过 PNA 杂交和 MALDI-TOF 质量分析结合定量检测单碱基突变。

Quantitative detection of single base mutation by combining PNA hybridization and MALDI-TOF mass analysis.

机构信息

R&D Center, Diatech Korea Co. Ltd., Seoul 138-200, Republic of Korea.

出版信息

Chem Commun (Camb). 2013 May 8;49(36):3754-6. doi: 10.1039/c3cc00070b. Epub 2013 Mar 27.

Abstract

Peptide nucleic acid (PNA) probes were designed to bind to the internal reference sequence and the single base mutation sequence within PCR-amplified DNA templates. PNAs hybridized to the target sequences on DNA were analyzed using MALDI-TOF mass spectrometry. Accurate quantification of the relative amount of mutant DNA was reproducibly demonstrated.

摘要

肽核酸（PNA）探针被设计为与 PCR 扩增的 DNA 模板中的内部参照序列和单个碱基突变序列结合。MALDI-TOF 质谱分析杂交到 DNA 靶序列上的 PNA。准确地定量了突变 DNA 的相对量，并且可重复性良好。