[完全切除术后胃肠道间质瘤患者复发、转移及预后相关生物标志物的研究]

[Study on associated biomarkers influencing recurrence, metastasis and prognosis in patients with gastrointestinal stromal tumors after complete resection].

作者信息

Fang Yu-jing, Zhang Rong-xin, Luo Rong-zhen, Ou Qing-jian, Wan De-sen, Pan Zhi-zhong, Wu Xiao-jun

机构信息

Department of Colorectal Surgery, Cancer Center, Sun Yat-sen University, State Key Laboratory in Southern China, Guangzhou 510060, China.

出版信息

Zhonghua Wei Chang Wai Ke Za Zhi. 2013 Mar;16(3):242-6.

PMID:23536344

Abstract

OBJECTIVE

To explore the associated biomarkers influencing recurrence, metastasis and prognosis in patients with gastrointestinal stromal tumors (GIST) after complete resection.

METHODS

Tumor tissue samples of 148 patients with GIST undergoing complete resection from January 1990 to December 2008 in Sun Yat-sen University Cancer Center were collected. The expressions of Ki-67, E-cadherin, MMP7, CD44, nm23, P53, survivin, Cyclin D1, COX-2, and VEGF in tumor tissue samples were detected by tissue microarray and immunohistochemistry (IHC). The association of above factors expressions with recurrence, metastasis and prognosis was examined.

RESULTS

Log-rank test showed that Ki-67, E-cadherin, MMP7, CD44, P53 and survivin were associated to disease-free duration after complete GIST resection (all P<0.05), and the Ki-67, E-cadherin, P53 and survivin were associated to overall survival (all P<0.05). Cox multivariate analysis revealed that disease-free survival was associated with Ki-67, CD44 and P53 (all P<0.05), and the overall survival was only associated with Ki-67 (P<0.05).

CONCLUSION

Ki-67, CD44 and P53 are closely associated with recurrence and metastasis after complete GIST resection, and Ki-67 can predict the prognosis of GIST.

摘要

目的

探讨影响胃肠道间质瘤（GIST）患者完全切除术后复发、转移及预后的相关生物标志物。

方法

收集1990年1月至2008年12月在中山大学肿瘤防治中心接受完全切除的148例GIST患者的肿瘤组织样本。采用组织芯片和免疫组织化学（IHC）检测肿瘤组织样本中Ki-67、E-钙黏蛋白、基质金属蛋白酶7（MMP7）、CD44、nm23、P53、生存素、细胞周期蛋白D1、环氧合酶-2（COX-2）和血管内皮生长因子（VEGF）的表达。检测上述因素表达与复发、转移及预后的相关性。

结果

Log-rank检验显示，Ki-67、E-钙黏蛋白、MMP7、CD44、P53和生存素与GIST完全切除术后的无病生存期相关（均P<0.05），且Ki-67、E-钙黏蛋白、P53和生存素与总生存期相关（均P<0.05）。Cox多因素分析显示，无病生存期与Ki-67、CD44和P53相关（均P<0.05），总生存期仅与Ki-67相关（P<0.05）。