Department of Agricultural Biology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju, Republic of Korea.
Pest Manag Sci. 2013 May;69(5):576-81. doi: 10.1002/ps.3497. Epub 2013 Mar 27.
To date, emphasis has been placed on studying the virulence of Isaria fumosorosea conidia rather than the virulence of their blastospores. In the present study, a comparison was made of the efficacy of blastospores of I. fumosorosea (SFP-198) versus conidia against greenhouse whitefly (GWF) Trialeurodes vaporarioum nymphs and thermotolerance at 50 °C. To improve thermotolerance and potency, the blastospores were suspended in corn oil with a wetting and an insect-filming agent.
Blastospores had similar efficacy against GWF nymphs to conidia in glasshouse conditions but had less thermotolerance than conidia. However, blastospores in corn oil had increased thermotolerance, comparable with that of conidia in corn oil. Application of SFP-198 blastospore oil-based formulation containing isotridecyl alcohol ethoxylated-3EO (TDE-3) and sodium alginate (SA) as adjuvants showed enhanced efficacy (95.7% mortality), compared with the SA-free oil formulation (72.8%) in 10 days.
SFP-198 blastospores can be used as an effective biological control agent, given their virulence against GWF, the enhanced thermotolerance in corn oil and the oil-based formulation studies to enhance their efficacy. Blastospores can be easily produced in liquid cultures in less than 4 days, which is significantly shorter than using traditional solid cultures for conidial production.
迄今为止，人们一直强调研究虫草菌分生孢子的毒力，而不是其芽生孢子的毒力。在本研究中，比较了虫草菌（SFP-198）芽生孢子与分生孢子对温室粉虱（GWF）烟粉虱若虫的防治效果和 50°C 时的耐热性。为了提高耐热性和药效，将芽生孢子悬浮在添加润湿剂和昆虫成膜剂的玉米油中。
在温室条件下，芽生孢子对 GWF 若虫的防治效果与分生孢子相似，但耐热性不如分生孢子。然而，玉米油中的芽生孢子耐热性增强，与玉米油中的分生孢子相当。含有异十三醇聚氧乙烯醚 3EO（TDE-3）和海藻酸钠（SA）作为助剂的 SFP-198 芽生孢子油基制剂的应用，与不含 SA 的油制剂（72.8%）相比，在 10 天内表现出更高的药效（95.7%死亡率）。
SFP-198 芽生孢子可以作为一种有效的生物防治剂，因为其对 GWF 的毒力、在玉米油中的增强耐热性以及油基制剂研究都可以提高其药效。芽生孢子可以在不到 4 天的时间内通过液体培养轻松生产，这明显短于使用传统的固体培养生产分生孢子的时间。
