Ishizuka Kazuo
HREM Research Inc., Matsukazedai, Higashimatsuyama, Saitama 355-0055, Japan.
Microscopy (Oxf). 2013 Jun;62 Suppl 1:S109-18. doi: 10.1093/jmicro/dft005. Epub 2013 Mar 27.
The iterative wave function reconstruction (IWFR) is one type of in-line holography and retrieves a complex wave function from a set of through-focus images. We have verified that the IWFR provides an extremely good estimate of an atomic-resolution exit wave function (EWF) simply from the Fourier transforms of observed intensities. Thus, the first guess of the EWF using only five images gives all the features of the final result, and the convergence of the IWFR is very quick. The IWFR accepts a wide variety of defocus step, and the total defocus span or the defocus step is not essential. The absolute defocus can be estimated by propagating the EWF to the plane where the propagated wave function gives the minimum amplitude variation. Even when there is some error in the spherical aberration coefficient, the EWF suffers from only the aberrations due to the estimation error. The residual error may be adjusted on the reconstructed complex wave function. With the development of a stable microscope it becomes more realistic to routinely record multiple images with good quality, which allows advanced image processing such as the IWFR. Such an exit wave reconstruction is also desirable to investigate a phase object using a Cs-corrected microscope, since the intentionally introduced aberrations to amplify the phase object contrast are desirable to be eliminated by post-processing.
迭代波函数重建(IWFR)是一种同轴全息术,可从一组离焦图像中检索复波函数。我们已经验证,IWFR仅通过观察强度的傅里叶变换就能对原子分辨率出射波函数(EWF)提供极其良好的估计。因此,仅使用五幅图像对EWF进行的首次猜测就给出了最终结果的所有特征,并且IWFR的收敛非常快。IWFR接受各种各样的离焦步长,总离焦跨度或离焦步长并不重要。可以通过将EWF传播到传播波函数振幅变化最小的平面来估计绝对离焦。即使球差系数存在一些误差,EWF也仅受估计误差引起的像差影响。残余误差可以在重建的复波函数上进行调整。随着稳定显微镜的发展,常规记录高质量的多幅图像变得更加现实,这使得诸如IWFR之类的高级图像处理成为可能。这种出射波重建对于使用Cs校正显微镜研究相位物体也是可取的,因为通过后处理消除有意引入的用于放大相位物体对比度的像差是可取的。