Department of Experimental Diagnostics and Therapy of Tumors, Russian Cancer Center, Moscow, Russia.
Cancer Gene Ther. 2013 Apr;20(4):237-41. doi: 10.1038/cgt.2013.11. Epub 2013 Mar 29.
Previous studies have demonstrated functional roles for microRNAs (miRNAs) in various aspects of normal and malignant hematopoiesis, including lineage commitment, differentiation, apoptosis and maturation. In vivo delivery of naked DNA, oligonucleotides and miRNAs is complicated by their low stability, rapid degradation and inefficient delivery into target cells. In our experiments, we used a new type of polymer carriers to monitor the effects of miR-155 and antago-miR-155 on the morphology and genetics of Kasumi-1 cells. We obtained platelet-like cells from leukemic cells, and detected the expression of platelet marker genes after transfection with antago-miR-155. Our findings suggest that administration of miR mimics or antago-miRs as therapeutic agents is a desirable goal for future treatment of hematologic malignancies and that polymer-based carriers for the delivery of miR mimics or antago-miRs may provide a solution to the challenges of standard miR delivery approaches.
先前的研究已经证明了 microRNAs（miRNAs）在正常和恶性造血的各个方面的功能作用，包括谱系承诺、分化、凋亡和成熟。裸 DNA、寡核苷酸和 miRNAs 的体内递送受到其低稳定性、快速降解和低效递送至靶细胞的影响而变得复杂。在我们的实验中，我们使用了一种新型聚合物载体来监测 miR-155 和 antago-miR-155 对 Kasumi-1 细胞形态和遗传学的影响。我们从白血病细胞中获得了类血小板细胞，并在转染 antago-miR-155 后检测了血小板标记基因的表达。我们的研究结果表明，作为治疗剂的 miR 模拟物或 antago-miRs 的给药是未来治疗血液恶性肿瘤的理想目标，并且用于递送 miR 模拟物或 antago-miRs 的聚合物载体可能为标准 miR 递送来提供解决方案。
