酶固定在纤维蛋白上。

Enzyme immobilization on fibrin.

作者信息

Dillon J G, Wade C W, Daly W H

出版信息

Biotechnol Bioeng. 1976 Jan;18(1):133-9. doi: 10.1002/bit.260180112.

Abstract

The following conclusions can be drawn concerning the utilization of fibrin to immobilized enzyme systems. Fibrin can be used both as a powder or membrane, to covalently immobilize trypsin with retention of activity. Carbon-14 labeled trypsin can be used to estimate the amount of immobilized enzyme on a proteinaceous support. Significant amounts of noncovalently coupled (adsorbed) enzyme are present on the surface of the support. Esterase activity of the immobilized labeled trypsin was inversely proportional to the amount of attached enzyme. Optimum TAME hydrolysis occurred at pH 8-8.4. The storage stability of trypsin was enhanced. Inhibition of trypsin esterase activity occurred at substrate concentrations greater than 30mM.

摘要

关于纤维蛋白在固定化酶系统中的应用，可得出以下结论。纤维蛋白既可以用作粉末，也可以制成膜，用于共价固定胰蛋白酶并保留其活性。碳 - 14标记的胰蛋白酶可用于估计蛋白质载体上固定化酶的量。在载体表面存在大量非共价偶联（吸附）的酶。固定化标记胰蛋白酶的酯酶活性与附着酶的量成反比。在pH 8 - 8.4时发生最佳的对甲苯磺酰-L-精氨酸甲酯（TAME）水解。胰蛋白酶的储存稳定性增强。在底物浓度大于30mM时，胰蛋白酶酯酶活性受到抑制。