Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Annu Rev Chem Biomol Eng. 2013;4:259-88. doi: 10.1146/annurev-chembioeng-061312-103312. Epub 2013 Mar 27.
We present here a broad overview of the field of metabolic engineering, describing in the first section the key fundamental principles that define and distinguish it, as well as the technological and intellectual developments over the past approximately 20 years that have led to the current state of the art. Discussion of concepts such as metabolic flux analysis, metabolic control analysis, and rational and combinatorial methods is facilitated by illustrative examples of their application drawn from the extensive metabolic engineering literature. In the second section, we present some of the rapidly emerging technologies that we think will play pivotal roles in the continued growth of the field, from improving production metrics to expanding the range of attainable compounds.
我们在这里对代谢工程领域进行了全面概述，在第一部分中描述了定义和区分该领域的关键基本原则，以及过去大约 20 年来导致该领域达到目前水平的技术和知识发展。通过从广泛的代谢工程文献中提取的应用实例，促进了对代谢通量分析、代谢控制分析和合理组合方法等概念的讨论。在第二部分，我们介绍了一些我们认为将在该领域的持续发展中发挥关键作用的快速涌现技术，从提高生产指标到扩大可获得化合物的范围。
