Chadwick R W, George S E, Chang J, Kohan M J, Dekker J P, Long J E, Duffy M C
Health Effects Research Laboratory Environmental Research Center, U.S. Environmental Protection Agency Research, Triangle Park, N.C. 27711.
Cancer Lett. 1990 Jun 30;52(1):13-9. doi: 10.1016/0304-3835(90)90071-5.
Comparative intestinal nitroreductase, azo reductase, beta-glucuronidase, dechlorinase and dehydrochlorinase activities in young male Fischer 344 rats and young male CD-1 mice were measured in vitro while the comparative biotransformation of 2,6-dinitrotoluene to mutagenic metabolites was determined in vivo. The mice, which exhibit a high spontaneous incidence of hepatomas, had markedly greater nitroreductase activity and metabolized significantly more 2,6-dinitrotoluene to mutagenic metabolites than did Fischer 344 rats, which show a low incidence of liver tumors. Results of this study indicate that species differences in the incidence of hepatomas may be influenced by microbial flora and/or the biotransformation of xenobiotics in the G.I. tract.
在体外测量了年轻雄性Fischer 344大鼠和年轻雄性CD-1小鼠的肠道硝基还原酶、偶氮还原酶、β-葡萄糖醛酸酶、脱氯酶和脱氯化氢酶的活性，同时在体内测定了2,6-二硝基甲苯向诱变代谢物的比较生物转化。肝癌自发发生率高的小鼠，其硝基还原酶活性明显更高，并且与肝癌发生率低的Fischer 344大鼠相比，将显著更多的2,6-二硝基甲苯代谢为诱变代谢物。本研究结果表明，肝癌发生率的种属差异可能受微生物菌群和/或胃肠道中外源生物的生物转化影响。
