Genomic Instability Group, Spanish National Cancer Research Centre, Madrid 28029, Spain.
Cell Rep. 2013 Apr 25;3(4):1105-16. doi: 10.1016/j.celrep.2013.03.009. Epub 2013 Mar 28.
DNA replication is facilitated by multiple factors that concentrate in the vicinity of replication forks. Here, we developed an approach that combines the isolation of proteins on nascent DNA chains with mass spectrometry (iPOND-MS), allowing a comprehensive proteomic characterization of the human replisome and replisome-associated factors. In addition to known replisome components, we provide a broad list of proteins that reside in the vicinity of the replisome, some of which were not previously associated with replication. For instance, our data support a link between DNA replication and the Williams-Beuren syndrome and identify ZNF24 as a replication factor. In addition, we reveal that SUMOylation is widespread for factors that concentrate near replisomes, which contrasts with lower UQylation levels at these sites. This resource provides a panoramic view of the proteins that concentrate in the surroundings of the replisome, which should facilitate future investigations on DNA replication and genome maintenance.
DNA 复制是由多种因素促进的，这些因素集中在复制叉附近。在这里，我们开发了一种将新生 DNA 链上的蛋白质与质谱（iPOND-MS）相结合的方法，从而能够全面蛋白质组学表征人类复制体和复制体相关因子。除了已知的复制体成分外，我们还提供了广泛的位于复制体附近的蛋白质列表，其中一些以前与复制无关。例如，我们的数据支持 DNA 复制与威廉姆斯综合征之间的联系，并鉴定出 ZNF24 是一种复制因子。此外，我们还揭示了在靠近复制体的因子中 SUMO 化是广泛存在的，而在这些位点上 UQ 化水平较低。该资源提供了一个集中在复制体周围的蛋白质全景图，这应该有助于未来对 DNA 复制和基因组维护的研究。
