Kumari P, Basal N, Singh A K, Rai V P, Srivastava C P, Singh P K
Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India.
Genet Mol Res. 2013 Mar 13;12(3):3540-50. doi: 10.4238/2013.March.13.12.
The genetic diversity among 28 pea (Pisum sativum L.) genotypes was analyzed using 32 simple sequence repeat markers. A total of 44 polymorphic bands, with an average of 2.1 bands per primer, were obtained. The polymorphism information content ranged from 0.657 to 0.309 with an average of 0.493. The variation in genetic diversity among these cultivars ranged from 0.11 to 0.73. Cluster analysis based on Jaccard's similarity coefficient using the unweighted pair-group method with arithmetic mean (UPGMA) revealed 2 distinct clusters, I and II, comprising 6 and 22 genotypes, respectively. Cluster II was further differentiated into 2 subclusters, IIA and IIB, with 12 and 10 genotypes, respectively. Principal component (PC) analysis revealed results similar to those of UPGMA. The first, second, and third PCs contributed 21.6, 16.1, and 14.0% of the variation, respectively; cumulative variation of the first 3 PCs was 51.7%.
利用32个简单序列重复标记对28个豌豆（Pisum sativum L.）基因型的遗传多样性进行了分析。共获得44条多态性条带，每个引物平均有2.1条带。多态性信息含量范围为0.657至0.309，平均为0.493。这些品种间的遗传多样性变异范围为0.11至0.73。基于杰卡德相似系数，采用非加权算术平均法（UPGMA）进行聚类分析，结果显示有两个不同的聚类，即聚类I和聚类II，分别包含6个和22个基因型。聚类II进一步分为两个亚聚类，即IIA和IIB，分别有12个和10个基因型。主成分（PC）分析结果与UPGMA分析结果相似。第一、第二和第三主成分分别贡献了21.6%、16.1%和14.0%的变异；前三个主成分的累积变异为51.7%。
